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BIOTEC WP Members

Thai Project Leader

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WP7 Project management & dissemination and exploitation

Nitsara KAROONUTHAISIRI, PhD
Experienced Researcher (ER-48)

Dr. Karoonuthaisiri is currently a Director of Biosensing Technology Research Unit at BIOTEC, Thailand. She finished her B.S. in Chemical Engineering (honors with distinctions, departmental valedictorian) from Columbia University (NY, USA) and her M.S. and Ph.D. in Chemical Engineering from Stanford University (CA, USA) in 2004.

In 2007, she established Microarray Laboratory where her research team employs microarray technology to study black tiger shrimp, an economically important export for Thailand, and develop antibody arrays for diagnostics. Her antibody array for foodborne pathogen detection won the 2010 Invention Award, while her research on the black tiger shrimp won the 2014 Research Award from the National Research Council of Thailand. She published over 40 peer-reviewed scientific papers and filed 14 patents. She was awarded “Outstanding Young Technologist of 2011” from the Foundation for the Promotion of Science and Technology under the Patronage of His Majesty the King, “2009 For Women in Science” fellowship from UNESCO-L’Oreal Thailand. In 2011, she was featured in Nature News in an article “Homecoming Queen”.

In 2011-2013, she took a sabbatical at Queen’s University Belfast (UK) under a Marie Curie Fellowship from the EU FP7. She was the founding co-chair of the Global Young Academy which aims to empower and mobilize young scientists to address issues of particular importance to early career scientists. She was honored as Young Global Leader 2013 by WEF. 

Other Members

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Wanilada RUNGRASSAMEE, PhD
Experienced Researcher (ER-36)

Dr. Rungrassamee completed her doctoral degree in Microbiology from University of Massachusetts Amherst. Her expertise is in the area of molecular microbiology including gene expression analysis and bacterial detection assays. Her research aims to develop sustainable approaches to lower a risk of disease outbreak in aquaculture through maintaining microbial balance in environment and inside host shrimp. She and her team focus on characterizing gut microbiota and their interactions to the host shrimp, along with development of DNA-based assays for multiplex gene expression analysis as a useful molecular tool for shrimp health evaluation.

Responsible WP(s) & Tasks:
WP1 Screening for promising beneficial bacteria for aquaculture.
WP3 Sharing knowledge on host-parasite interactions in invertebrates.
WP4 Determine stability and modulation of shrimp gut microbiome under different feed additives.
WP5 Determine transcriptomic gene expression and gut microbial population in shrimp intestine upon exposure to pathogen using direct next generation sequencing approaches.

 

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Rungnapa LEELATANAWIT, PhD
Experienced Researcher (ER-29)

Dr. Leelatanawit received her doctorate in biotechnology from Chulalongkorn University, Thailand. Her expertise covers molecular biology, shrimp biology, and shrimp reproduction. She attempts to address the poor reproductive maturation problem in the black tiger shrimp (Penaeus monodon) in captivity over 16 years using several molecular tools such as EST library construction, microarray analysis, gene expression analysis, recombinant protein construction, and protein expression analysis. Currently, her research interest focuses on the effects of feeds on sperm quality and ovarian development of shrimp.

Responsible WP(s) & Tasks:
WP2 Extracting genomic DNA from shrimp tissues and preparing DNA library for sequencing. Annotation of shrimp genome sequence, including gene identification and mapping introns and exons borders, promoter and UTR for the genes in pathways involved with growth and reproduction such as Gonadotrophin-releaseing homone (GnRH) signaling pathway, progesterone-mediated oocyte maturation pathway, and P13K-AKT signaling pathway.Learning about knowledge of genomics and data utilization and Preparing a manuscript on the genome sequence to disseminate the knowledge as well.

 

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Sage CHAIYAPECHARA, PhD
Experienced Researcher (ER-38)

Dr. Chaiyapechara graduated from School of Aquatic and Fishery Sciences, University of Washington. His interests are on the involvement of bacteria in aquaculture environments, particularly in regards to the interaction between the gut microbiota and the health of the host animal. Recent research focused on characterizing bacteria associated with the early developmental stages of shrimp (L. vannamei) and the rearing environmental in hatcheries. His goal was to apply the knowledge of host-bacterial interaction into controlling diseases and maintaining optimum health for aquatic animals.

Responsible WP(s) & Tasks:
WP4 Intestinal bacterial population analysis in black tiger shrimp postlarvae. Determine stability and modulation of shrimp gut microbiome under different feed additives.

WP5 Examine the interaction between a host shrimp to their gut microbial community.

 

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Wananit WIMUTTISUK, PhD
Experienced Researcher (ER-31)

Dr. Wimuttisuk received her doctorate in biochemistry from Brown University. Her expertise covers protein-protein interactions, gene expression analysis, protein degradation pathway, and shrimp reproduction. She is a pioneer in the eicosanoid biosynthesis pathway in Penaeus monodon and determining its role in the P. monodon ovary development. Currently, her research interest focuses on the roles of fatty acids and eicosanoids in shrimp sperm development and shrimp immunity.

Responsible WP(s) & Tasks:
WP2 Annotation of shrimp genome sequence, including gene identification and mapping introns and exons borders, promoter and UTR for the genes in the eicosanoid biosynthesis pathway, steroid biosynthesis pathway, fatty acid b-oxidation and fatty acid biosynthesis pathway.
WP3 Investigating P. monodon immunology in response to WSSV infection. Learning about host-parasite interactions in invertebrates. Exploring the potential to use lipids and lipid derivatives as biomarkers for P. monodon growth and sexual maturation. Learning techniques on defective interfering genome and planning subsequent experiments as proposed in WP3.4 and WP3.5.

 

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Tanaporn UENGWETWANIT, PhD
Experienced Researcher (ER-30)

Dr. Uengwetwanit received her Bachelor degree in Pharmacy from Silpakorn University and Master degree in Bioinformatics from King Mongkul University Thonburi, Thailand. She received her Ph.D. in Pharmaceutical Science from Martin Luther University Halle-Wittenberg, Germany in 2014. Her research work spans a variety of areas including computer-aided drug discovery/design and bioinformatics. Currently, she has participated in projects involving shrimp genome, transcriptome and microbiome.

Responsible WP(s) & Tasks:
WP2 Design and analysis of genome sequencing data. Building a website for providing shrimp genome database for the study of Penaeus monodon. 
WP4 Analysis of 16S sequencing data to determine microbiome in different conditions.
WP5 Analysis of sequencing data to determine transcriptomic gene expression in shrimp intestine upon exposure to pathogen.

 

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Khaow TONSOMBOON, PhD
Experienced Researcher (ER-47)

Dr. Tonsomboon received his doctorate in Engineering from Cambridge University. His expertise covers tissue engineering, biomaterials, biomimicry and biomechanics. He is also an enthusiastic science communicator, taking part in a variety of outreach scientific activities. Recently, he won the first edition of FameLab Thailand, a science communication competition, and represented Thailand in the international round at the Cheltenham Science Festival, UK. Currently, He is hosting a TV program called ‘Wow Wit’ broadcasted on a national TV station, communicating research activities conducted by the National Science and Technology Development Agency (NSTDA) to the public and relevant stakeholders.    

Responsible WP(s) & Tasks:
WP7 Dissemination and networking. Outreach to the scientific community.

 

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Pilasinee TEPTADA, MS
Administrative Staff (ADM-46)

Ms. Teptada received her first M.S. degree in Biochemistry & Molecular Biology from Georgetown University and her second M.S. in Management from Rensselaer Polytechnic Institute. She is currently working as a Project Analyst in the External Grant Administrative Office at BIOTEC providing support for researchers as they conceptualize, develop, and write their proposals to international funding agencies, as well as assisting researchers in obtaining and managing research grants funded by international funding sources.   

Responsible WP(s) & Tasks:
WP6 Maximizing knowledge sharing strategy by implementing effective staff exchange procedures between participating organizations. Building future collaborations to ensure self-sustainability. Integrating knowledge and project management to ensure future project success.

 

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Yuwares MALILA, PhD
Experienced Researcher (ER)

Dr. Malila received her doctoral degree in Food Science from Michigan State University, USA. She has an experience in transcriptome profiling of skeletal muscle between normal and pale, soft and exudative (PSE) turkey meat using microarray and RNA sequencing platforms. Her expertise also covers calcium homeostasis and postmortem metabolism in skeletal muscle associated with meat quality. Currently, her research focuses on quality determination of muscle foods, as well as identification of molecular pathways associated with development of wooden breast and white striping defects in chicken breast meat. 

Responsible WP(s) & Tasks:
WP4 Gut microbiota

 

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Atikorn PANYA, PhD
Experienced Researcher (ER-41)

Dr. Panya received his doctorate in food chemistry from University of Massachusetts, Amherst. His expertise covers food lipids, proteins and colloids, lipid oxidation and antioxidant mechanisms in food matrices. Currently, his research interest focuses on developing analytical methods for volatile, semi- and non-volatile compounds using several GC-MS/MS techniques.

Responsible WP(s) & Tasks:
WP5 Host-microbe Interactome

 

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Rinrada SUNTIVICH, PhD
Experienced Researcher (ER-42)

Dr. Suntivich received a Ph.D. in Chemistry from The Ohio State University. Her research experience covers peptide solid phase synthesis, high-throughput screening and mass spectrometry. She is currently working on using metabolomics as a tool to explore natural product research.

Responsible WP(s) & Tasks:
WP5 Optimization of tissue sample preparation and extraction for metabolite analysis. Metabolite profiling in shrimp gut in non- and pathogen exposure trials, e.g. target metabolomics for quorum sensing pathway.

 

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Surapun TEPAAMORNDECH, PhD
Experienced Researcher (ER-40)

Dr. Tepaamorndech joined Food Biotechnology Research Unit, BIOTEC, NSTDA in August, 2015. He obtained a Ph.D. degree in Integrative Genetics and Genomics at University of California Davis in 2015. He has primarily focused on metagenomics of bioflocs and the effect of the environmental bacteria on shrimp gut microbiome and immunity in Safe-Aqua project.

Responsible WP(s) & Tasks:
WP4 Determine stability and modulation of shrimp gut microbiome under different feed additives.

 

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Umaporn UAWISETWATHANA, PhD
Experienced Researcher (ER-45)

Dr. Uawisetwathana received her doctorate in biology from Queen’s University Belfast. Her expertise covers –Omics analysis, namely transcriptomics and metabolomics, for food and agriculture. Her researches are involved in development of antibody array for detection of food borne pathogens, fabrication of an in-house cDNA microarray for the black tiger shrimp and establishment of rice metabolomics for BPH-resistant traits. She is a part of establishing metabolomics core facility in BIOTEC. Currently, her research interest focuses on metabolic responses of rice and shrimp to biotic and abiotic stresses for improving sustainable production. 

Responsible WP(s) & Tasks:
WP5 Optimizing protocols on targeted quorum sensing metabolite profiles for shrimp gut microbiota. Analyzing high resolution mass spectrometry-based metabolite profiles in shrimp gut in non- and pathogen exposure trials. Learning data analysis for shrimp-gut microbiota interactome. Training on how to identify metabolite profiles high-resolution mass spectrometer. Workshop on Metabolomics and its Applications in Aquaculture and Food Security at BIOTEC.

 

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Virak VISUDTIPHOLE, PhD
Experienced Researcher (ER-27)

Dr. Visudtiphole received his doctorate degree in biochemistry from University of Newcastle upon Tyne. His research interests are aqua-feed and nutrition, focusing on fish meal and oil replacement through microbe utilisation. He also studies stress-related genes and other health parameters that can be accurate indicators for growth performance of shrimp applicable to the feed research and farming industry. His current ongoing researches are application of marine microbes in feed to improve health of larval shrimp; effects of prebiotic feed additives and  on shrimp health.

Responsible WP(s) & Tasks:
WP4 Effect of Aurantiochytriumsupplement on shrimp’s lipidomics.
WP5 Bioenergetic flux analysis of shrimp hemocytes under abiotic stress e.g. salinity and temperature.

 

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Suganya YONGKIETTRAKUL, PhD
Experienced Researcher (ER-43)

Dr. Yongkiettrakul received her Ph.D. degree in biochemistry from The Ohio-State University. Her expertise covers protein-protein interaction, drug-target interaction, and analysis and application of biomolecules.  Her research focuses on the discovery of biological targets and drug-resistant mechanism of human pathogens, including malaria, influenza A viruses and bacterial Streptococcal bacteria.  She recently has employed bacterial surrogate model to understand the effect of antifolate drugs in folate biosynthesis pathway of Plasmodium spp.  Currently, she is establishing LCHRMS-based metabolomics approaches to investigate metabolic hotspots involved in molecular mechanism of drug resistance and pathogenesis of zoonotic bacteria. 

Responsible WP(s) & Tasks:
WP5 Development of LCHRMS-based metabolomic approaches for host-microbe interaction.Learning integrative analysis of omics data.

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Sopacha ARAYAMETHAKORN, MS
Early Stage Researcher (ESR-39)
 

Ms. Arayamethakorn graduated her B.S. in biochemistry and M.S. in biotechnology from Chulalongkorn University. Her expertise is in area of biochemistry and molecular biology. Her research interests are DNA-based high throughput technology for multiplex gene expression, bacterial identification and protein analysis. Currently, her research focuses on gut microbial diversity analysis in the black tiger shrimp and the pacific white shrimp under different feed additives, and development of DNA-based bead array for multiplex gene expression analysis.

Responsible WP(s) & Tasks:
WP1 Determination of feed additive effects to shrimp gut microbiota analysis.
WP4 Sample preparation for intestinal population analysis.
WP5 Learning about direct RNA sequencing approach.

 

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Phimsucha BUNPHIMPAPHA,MSc
Early Stage Researcher (ESR-37)

Ms. Bunphimpapha received her master degree in biology from Srinakharinwirot University. Her thesis was titled "Production of monoclonal antibodies specific to Aeromonas caviae, gene isolation and expression of lipase/esterase from metagenomic DNA library of thermotolerant microorganisms". Currently, her research at BIOTEC was on examining the bacterial community in the shrimp gut from hatcheries. Her skills includes various methods in Molecular biology such as DNA & RNA extraction, cDNA synthesis, real time PCR, clone libraries, Barcode pyrosequencing, Denaturing Gradient Gel Electrophoresis (DGGE), Automated Ribosomal Intergenic Spacer Analysis (ARISA), Fluorescence in situ hybridization (FISH), and Immunohistochemistry (IHC).

Responsible WP(s) & Tasks:
WP4 Intestinal bacterial population analysis in black tiger shrimp postlarvae. Determine stability and modulation of shrimp gut microbiome under different feed additives.

 

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Pacharawan DEENARN, MSc
Early Stage Researcher (ESR-32)

Ms. Deenarn received her master degree in biotechnology from Chulalongkorn University, Thailand. Her work experiences include antimicrobial drug discovery, protein expression in E. coli system, protein purification, real-time PCR analysis and other molecular techniques. Her main research focuses on the role of fatty acid biosynthesis and transport in the P. monodon reproductive development.

Responsible WP(s) & Tasks:
WP3 Analyzing of hemocyanin-derived antimicrobial peptides. Investigating cell death in shrimp after pathogenic infection. Computer modeling for domain analysis.

 

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Surasak JIEMSUP, BSc
Early Stage Researcher (ESR-44)

Mr. Jiemsup received his BSc in Microbiology from Ubonratchatani University, Thailand. My work focuses on the application of mass spectrometry-based analysis. I have experienced in protein purification, proteomics application, and metabolite detection. I developed LC-ESI/MS approaches for the determination of antifolate effect on folate biosynthesis pathway.  Currently, I am developing targeted metabolomics for understanding the role of arginine biosynthesis pathway on bacterial pathogenesis.

Responsible WP(s) & Tasks:
WP5 Development of LCHRMS-based analysis for metabolomics application.  

 

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Thapanee PRUKSATRAKUL, MSc
Early Stage Researcher (ESR-34)

Ms. Pruksatrakul received her master degree in applied analytical and inorganic chemistry program from Mahidol University, Thailand. Her core skill sets include flow injection analysis (FIA), extraction of short chain fatty acid in palm oil mill effluent, chromatography techniques (HPLC, GC and CE), and isolation of natural product (LC-MS). Aside from working as part of the natural product discovery team at BIOTEC, she will be the driving force at setting up analysis of polyunsaturated fatty acids and eicosanoids in the P. monodon using UHPLC-HRMS/MS. 

Responsible WP(s) & Tasks:
WP3 Lipid profiling and high density lipoprotein analysis. Protocol development for the analysis of eicosanoids and fatty acid profiles in P. monodon tissues using UHPLC-HRMS/MS and other relevant techniques. Data analysis and data management from UHPLC-HRMS/MS results.

 

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Punsa TOBWOR, MSc
Early Stage Researcher (ESR-33)

Ms. Tobwor received her master degree in medical microbiology from Khonkaen University, Thailand. Her research expertise includes chemical extraction, HPLC analysis, real-time PCR analysis and other molecular techniques. Currently, her research interest focuses on the characterization of cyclooxygenase enzyme and its role in the P. monodon reproductive development. 

Responsible WP(s) & Tasks:
WP3 Characterization of hemocyanin-derived phenoloxidase activity. Protocol development for the analysis of P. monodon tissues using GC and GC/MS. Data analysis and data management from GC and GC-MS results.

 

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Pacharaporn ANGTHONG, PhD
Postdoctoral researcher

Dr. Angthong received her doctorate in biotechnology from Chulalongkorn University, Thailand. Her expertise covers molecular biology and shrimp gut immunity. Her research studies on the function of shrimp lectins and their involvement in gastrointestinal system of black tiger shrimp. Currently, her research focus on gut microbial community of black tiger shrimp under different growth stages

Responsible WP(s) & Tasks:
WP4 Prepare the samples for next generation sequencing and determine stability and modulation of shrimp gut microbiome under different feed additives.